Printer friendly version [26 pages] Microbiology laboratories commonly receive cerebrospinal fluid CSF or blood specimens from patients with meningitis, pneumonia, or unexplained febrile illness. Laboratories may also receive joint fluid, pleural fluid, or other sterile site specimens from these patients. Presumptive identification of N. Methods for confirmatory identification of N.
Transfusion Medicine Effects of Hemolysis on Clinical Specimens Hemolysis due to the breakdown of red blood cells is important to the laboratory because it can have an effect on laboratory results. The effects can be the result of products liberated from the red cells themselves, or due to interferences with laboratory analyzers.
This may vary from one test to another depending on the formulation of the reagent. Hemolysis can occur in vivo in the patientdue to a variety of medical conditions, including antigen-antibody reactions, hemolytic anemias, toxins and poisons, mechanical RBC rupture due to artificial heart valves, as well as treatments such as hemodialysis and the use of the heart-lung bypass machine.
Hemolysis can occur during suboptimal blood collection, or in vitro e. The amount of hemolysis needed to affect a test is dependent on the test being performed. In general, slight hemolysis has little effect on most tests; however, it will cause increased test results for specific tests like potassium and lactate dehydrogenase see table below.
Samples with slight hemolysis are analyzed and the results reported with a comment indicating the degree of hemolysis and the effect on the test result. Grossly hemolyzed samples can affect the results of many tests; therefore, a recollection will be requested for most grossly hemolyzed samples.
Notable examples of tests affected by hemolysis are found in the table below.Haptoglobin is a protein produced by the liver that the body uses to clear free hemoglobin (found outside of red blood cells) from circulation. A haptoglobin test is used primarily to help detect and evaluate hemolytic anemia when a person has symptoms such as jaundice, fatigue, weakness and red .
Table of Contents INTRODUCTION 2 Your role 2 BEFORE YOU BEGIN 3 Patient identification 3 Supplies 4 Special collection requirements and restrictions Label information.
Tissue Transglutaminase Antibody, IgA, is useful in diagnosing gluten-sensitive enteropathies, such as Celiac Sprue Disease, and an associated skin condition, dermatitis herpetiformis.
Hemolysis can be caused by: Shaking the tube too hard.
Using a needle that is too small. Pulling back too hard on a syringe plunger. Pushing on a syringe plunger too .
RBC: Red Blood Cell (erythrocyte) non-nucleated cells in blood, carry oxygen and CO2 on the hemoglobin. RBCs make up from % of the blood volume. HealthLab Blood Collection Tubes The alphabetical test listing section of this manual details specific specimen requirements including storage.